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CD8 (Leu2, MAL, p32, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2)

CD8 (Leu2, MAL, p32, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2)

Cat no: C2259-04A

Supplier: United States Biological
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Human CD8 is a heterodimeric protein consisting of an alpha and beta chain, each about 33kD. CD8 is expressed on most thymocytes and on about one third of peripheral blood T cells. CD8 is a co-receptor involved in antigen recognition and binds to the alpha 2 and alpha 3 domain of MHC Class I molecules (3, 4). Applications: Suitable for use in ELISA, Western Blot, Flow Cytometry and Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: Capture Flow Cytometry: 5x10e5 cultured human HPB-MLT cells per tube were washed and pre-incubated with 20ul human IgG at 250ug/ml (to block non specific binding) after which they were incubated 45min on ice with 80ml of anti-CD8 antibody at a concentration of 5ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.67 log10 fluorescent units when compared to a Mouse IgG2a negative control at a similar concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2259-04A
Reactivities: Human
Hosts: Mouse
Applications: ELISA, Flow Cytometry, Immunocytochemistry, Western Blot
Size: 100ug
Form: Supplied as a liquid.
P type: Mab
Isotype: IgG2a
Purity: Purified
References: 1. A. Fischer, et al, (1983) Immunology 48:177-186. 2. R. Zamoyska, (1994) Immunity 1:243-246. 3. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 353-356. 4. J. Sun, et al, (1995) J Exp Med 182:1275-1280. 5. J Hirst, MS Robinson, et al.(2007) Traffic 8:594-604.
Additional info: Recognizes human CD8 alpha chain of approximately 33kD.

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