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CD8 (Leu2, MAL, p32, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2)

CD8 (Leu2, MAL, p32, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2)

Cat no: C2259-04B

Supplier: United States Biological
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Human CD8 is a heterodimeric protein consisting of an a and B chain, each about 33kD. CD8 is expressed on most thymocytes and on about one third of peripheral blood T cells. CD8 is a co-receptor involved in antigen recognition and binds to the a2 and a3 domain of MHC Class I molecules. Applications: Suitable for use in ELISA and Flow Cytometry. Other applications not tested. Recommended Dilution: ELISA: Detector Flow Cytometry: 5x10e5 cultured HPB-MLT cells per tube were washed and incubated 45 min on ice with 80ul of anti-CD8 antibody at 5ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.28 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2259-04B
Reactivities: Human
Hosts: Mouse
Applications: ELISA, Flow Cytometry
Size: 100ug
Form: Supplied as a liquid in 50 mM sodium phosphate pH 7.5, 100 mM potassium chloride, 150mM sodium chloride. No preservative added.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
References: 1. Leukocyte Typing II (E.L. Reinherz, et al, eds.) Springer Verlag, New York, (1986). R. Zamoyska, (1994) Immunity 1:243-246. Leukocyte Typing V ( S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 353- 356. J. Sun, et al, (1995) J Exp Med 182:1275-1280.
Additional info: Recognizes human CD8.

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