Human CD8 is a glycoprotein expressed on the surface of T cells that have specificity for antigens presented by MHC class I proteins. CD8 has been shown to mediate signal transduction and adhesion on a subset of cells within the T cell compartment and is critically involved in the development of T cells expressing MHC class I-restricted T cell receptor (TCR). Besides the T cell subset, CD8 is also expressed in thymocytes, bone marrow and NK cells. The differentiation of CD4/CD8 double- positive thymocytes into CD8 single-positive T cells requires appropriate signals from the TCR and the co-receptor CD8. Cytotoxic T lymphocytes (CTL) expressing the cell surface glycoprotein CD8 play an important role in immune protection against intracellular pathogens such as viruses. CD8 greatly enhances antigen recognition by CTL and is referred to as a co-receptor, since it binds to the same peptide-major histocompatibility complex class I molecules as the T-cell receptor (TCR).
Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 0.5-2ug/ml
Immunoprecipitation: 3-5ug/extract from 10e7 cells
Immunohistochemistry: 1:100 for 10min at RT
Staining of formalin-fixed tissues requires boiling tissues sections in 10mM citrate buffer, pH 6.0, for 10min followed by cooling at RT for 20min
Optimal dilutions to be determined by the researcher.
Positive Control:
Tonsil
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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