The human anaphylatoxin C5a is a 74-residue glycopolypeptide which is generated by proteolytic cleavage of the complement factor C5 in the course of complement activation. A variety of biological effects evoked by C5a could be demonstrated, rendering this molecule an important mediator of inflammation, with granulocytes and macrophages as the main target cells. All cellular responses to C5a are specifically mediated by interactions with the membrane bound C5a receptor (CD88), a seven transmembrane GTP binding-protein-coupled receptor that belongs to the rhodopsin supergene family. The C5a receptor is approximately 45kD.
Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Flow Cytometry: 1:10-1:1000
Immunohistochemistry (frozen and paraffin): 1:10-1:2000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Quick Search
Products 
