CD99-L2, Fc Chimera, Recombinant, Human (CD99 antigen-like 2)

CD99 antigen-like 2 (CD99-L2) is a 45kD type I transmembrane glycoprotein in the CD99 family of molecules (1-3). Mouse CD99-L2 cDNA encodes a 214 amino acid (aa) precursor with a 23 aa predicted signal sequence, a 116 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 54 aa cytoplasmic region (4). The ECD contains no N-linked glycosylation sites, but O-linked glycosylation is likely (1, 2). A long isoform with a 23 aa insert after aa 43 within the ECD is expressed in human, and probably in mouse (2, 5). Both long and short isoforms may have minor variants missing portions of the N-terminus of the mature protein (6). The ECD of mouse CD99-L2 short isoform shares 85%, 72% and 66% aa identity with rat, human, and bovine CD99-L2, respectively (3, 5). The mouse CD99 and CD99-L2 ECDs share only 31% aa identity, but both contain three conserved acidic motifs and are thought to originate from the same ancestral gene (1, 2). The nearly ubiquitous expression of CD99-L2 is similar to that of CD99. Mouse CD99-L2 shows highest in situ hybridization signals in neurons, cortical thymocytes, ganglia, ovarian granulosa cells, testis, and kidney, and detectable protein levels in lung, thymocytes, mouse leukocytes and vascular endothelial cells (2, 7). CD99-L2 expression on endothelial cells is reported to mediate cell aggregation and neutrophil or monocyte extravasation to inflamed tissue in vivo, while CD99 mediates lymphocyte extravasation as well (3, 7).\n\nSource: A DNA sequence encoding the extracellular domain of human CD99-L2 (Val 20-Ala 188; Accession# NP_113650) was fused to the Fc region of human IgG1 via a peptide linker. The chimeric protein was expressed in Chinese Hamster Ovary cells.\n\nMolecular Mass:\nThe recombinant human CD99-L2/Fc is a disulfide linked homodimeric protein. Based on N-terminal amino acid sequencing, the recombinant human CD99-L2/Fc starts at Val 20. Each monomer has a calculated molecular mass of approximately 44.5kD. The recombinant human CD99-L2 migrates as an approximately 65-75kD protein in SDS-PAGE under reducing conditions.\n\nEndotoxin: (same/less than) 1.0 EU per 1ug of the protein as determined by the LAL method.\n\nActivity:\nMeasured by its ability to bind biotinylated rmCD99-L2 in a functional ELISA type binding assay.\n\nStorage and Stability:\nLyophilized samples are stable for up to twelve months at -20 to -70 degrees C.\nUpon reconstitution, this protein can be stored under sterile conditions at 2-8 degrees C for one month or at -20 to -70 degrees C in a manual defrost freezer for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

C2444-16B

Size

50ug

Reactivities

Hum

Form

Lyophilized from a 0.2um filtered solution in PBS. Reconstitution: Add sterile PBS be added to the vial to prepare a working stock solution of 100ug/ml.

Purity

(same/more than) 95% as determined by SDS-PAGE under reducing conditions and visualized by silver stain.

References

1. Suh, Y.H. et al., 2003, Gene 307:63.\n2. Park, S.H. et al. (2005) Gene 353:177.\n3. Schenkel, A.R. et al., 2007, Cell Commun. Adhes. 14:227.\n4. Swissprot Accession # Q8TCZ2.\n5. Entrez Accession # EAW99391.\n6. Bixel, G. et al., 2007, Blood 109:5327.