CD99, Recombinant, Human (MIC2, p30/32MIC2, Ewing

CD99 is an integral membrane protein of 185 amino acids that is expressed highly on cells of the hematopoietic system and on Ewing tumor cells. CD99 has been implicated in cell adhesion and cell death and to participate through these processes in the differentiation of T-cell precursors at a critical stage of thymocyte differentiation when positive selection is known to occur (Bernard et al). Engagement of the CD99 molecule by CD99 monoclonal antibodies has been shown to induce homotypic aggregation of various cell types. CD99 is expressed constitutively on all peripheral blood T-cells and becomes further up-regulated upon cell activation . CD99 mediates TCR/CD3-dependent activation of resting peripheral blood T-cells and specifically induces the production of the Th1 cytokines TNF-alpha and IFN-gamma.\n\nRecombinant CD99 shows a band at 56kD on SDS-PAGE commassie blue stained gel.\n\nApplications: \nSuitable for use in ELISA, Western Blot and Inhibition Assays. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2444-14

Size

2ug

Form

Supplied as a liquid in 50mM Tris-Acetate, pH 7.5, 1mM EDTA, 20% glycerol.

Purity

Purified by chromatography.

References

1. CD99 costimulation up-regulates T cell receptor-mediated activation of JNK and AP-1. Yoon SS, Kim HJ, Kim TJ, Mol Cells 2004 Oct 31;18(2):186-91 2. Prevalence of CD99 protein expression in pancreatic endocrine tumours (PETs). Goto A, Niki T, Fukushima J, Histopathology 2004 Oct;45(4):384-92 3. Identification of cyclophilin A as a CD99-binding protein by yeast two-hybrid screening. Kim HJ, Chong KH, Lee JR, Immunol Lett 2004 Sep;95(2):155-9 4. Solution structure of the cytoplasmic domain of human CD99 type I. Kim HY, Kim YM, Park SH, Mol Cells 2004 Aug 31;18(1):24-9 5. Mouse CD99 participates in T-cell recruitment into inflamed skin. Bixel G, Kloep S, Petri B, Blood 2004 Nov 15;104(10):3205-13 6. Recognition of Mycoplasma hyorhinis by CD99-Fc molecule. Gazit R, Rechnitzer H, Katzenell A, Gonen-Gross T, Eur J Immunol 2004 Jul;34(7):2032-40.