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CDC2 (Cell Division Protein Kinase 1, Cell Division Control Protein 2 Homolog, Cyclin-dependent Kinase 1, CDK1, p34 Protein Kinase, CDK1)

CDC2 (Cell Division Protein Kinase 1, Cell Division Control Protein 2 Homolog, Cyclin-dependent Kinase 1, CDK1, p34 Protein Kinase, CDK1)

Cat no: C2549-50P1

Supplier: United States Biological
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CDC2 is a catalytic subunit of a protein kinase complex, called the M-phase promoting factor, that induces entry into mitosis and is universal among eukaryotes. In HeLa cells CDC2 is the most abundant phosphotyrosine-containing protein and its phosphotyrosine content is subject to cell cycle regulation. CDC2 gene is located on chromosome 10. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.5-1ug/ml Immunohistochemistry: 1-2ug/ml detects CDC2 in formalin fixed and paraffin embedded tissues and formalin or acetone fixed frozen tissues. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile 1.2% sodium acetate or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C2549-50P1
Reactivities: Human, Mouse, Chicken/Bird
Hosts: Mouse
Applications: Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a lyophilized powder from 1.2% sodium acetate, 2% BSA, 0.01% sodium azide. Reconstitute with 1ml sterile 1.2% sodium acetate or PBS.
P type: Mab
Isotype: IgG2a
Purity: Purified by affinity chromatography.
Additional info: Recognizes CDC2. Species Crossreactivity: human, mouse, chicken

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