CDC2/CDK1, a CDC2/CDK -type protein kinase, regulates cell cycle transitions G1 to S and G2 to M. CDC2/CDK1 is maintained in an inactive state by phosphorylation by kinases MYT1 and WEE1. The active kinase, called the M-phase promoting factor, consists of a heterodimer with cyclin B, and final activation at G2/M results from CDC25 phosphatase-mediated dephosphorylation of CDC2/CDK1. The drug Taxol has been shown to activate CDC2/CDK1 in MDA-MB-435 breast cancer cells, leading to cell cycle arrest at G2/M and to apoptosis. CDC2 has also been implicated in neurofibrillary degeneration in Alzheimer's disease and other neurodegenerative diseases.
Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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