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cdc2, phosphorylated (Thr14)

cdc2, phosphorylated (Thr14)

Cat no: C2549-48

Supplier: United States Biological
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The entry of eukaryotic cells into mitosis is regulated by cdc2 kinase activation, a process controlled at several steps including cyclin binding and phosphorylation of cdc2 at Thr161 (1). However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of cdc2 at Tyr15 and Thr14 (2). Phosphorylation at Thr14 and Tyr15 resulting in inhibition of cdc2 can be carried out by Wee1 and Myt1 protein kinases (3,4). The cdc25 phosphatase may be responsible for removal of phosphates at Thr14 and Tyr15 and subsequent activation of cdc2 (1,5). Applictions: Suitable for use in Western Blot. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C2549-48
Reactivities: Human, Non-Human Primate
Hosts: Rabbit
Applications: Western Blot
Size: 100ul
Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol, 0.02% sodium azide.
P type: Pab
Purity: Purified by Protein A affinity chromatography.
References: 1.Atherton-Fessler, S. et al. (1994) Mol. Biol. Cell. 5, 989-1001.Norbury, C. et al. (1991) EMBO. J. 10, 3321-3329.McGowan, C.H. and Russell, P. (1993) EMBO J. 12, 75-85.Wells, N.J. et al. (1999) J. Cell. Sci. 112, 3361-3371.Hunter, T. (1995) Cell 80, 225-236.
Additional info: Recognizes endogenous levels of human cdc2 (CDK1) only when phosphorylated at Thr14. Species crossreactivity: monkey and hamster. Species sequence homology: mouse, rat, bovine, D. melanogaster and xenopus, CDK2 and CDK3.

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