Home  >  Products  >  CDC2, phosphorylated (Thr161) (Cell Division Protein Kinase 1, Cell Division Control Protein 2 Homolog, Cyclin-dependent Kinase 1, CDK1, p34 Protein Kinase, CDK1)
CDC2, phosphorylated (Thr161) (Cell Division Protein Kinase 1, Cell Division Control Protein 2 Homolog, Cyclin-dependent Kinase 1, CDK1, p34 Protein Kinase, CDK1)

CDC2, phosphorylated (Thr161) (Cell Division Protein Kinase 1, Cell Division Control Protein 2 Homolog, Cyclin-dependent Kinase 1, CDK1, p34 Protein Kinase, CDK1)

Cat no: C2549-49X

Supplier: United States Biological
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Entry of all eukaryotic cells into M-phase of the cell cycle is regulated by activation of cdc2 kinase. Activation of cdc2 is controlled at several steps including cyclin binding and phosphorylation of threonine 161. The critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of tyrosine 15 and threonine 14. Phosphorylation at Tyr15 and inhibition of cdc2 is carried out by Wee1 and Myt1 protein kinases. Tyr15 dephosphorylation and activation of cdc2 is carried out by the cdc25 phosphatase. cdc2 activation requires phosphorylation at Thr161, which is attributed to the cdk-activating kinase CAK, a complex of cdk7 and cyclin H. Phosphorylation at Thr161 is also required for association with cyclin A. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:1000 Immunohistochemistry: 1:50-1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2549-49X
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Immunohistochemistry, Western Blot
Size: 50ug
Form: Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: 1. Ukomadu, C., et al., J. Biol. Chem. 278(7): 4840-4846 (2003). 2. Morris, M.C., et al., J. Biol. Chem. 277(26): 23,847-23,853 (2002). 3. Brown, N.R., et al., J. Biol. Chem. 274(13): 8746-8756 (1999). 4. Liu, T., et al., J. Biol. Chem. 279(6): 4507-4514 (2004).
Additional info: Recognizes endogenous levels of human cdc2 only when phosphorylated at Thr161. Species Cross-Reactivity: mouse, rat.

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