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cdc25A (Cell division cycle 25A isoform a, Cell division cycle 25A isoform b, Dual specificity phosphatase CDC25A, M phase inducer phosphatase 1)

cdc25A (Cell division cycle 25A isoform a, Cell division cycle 25A isoform b, Dual specificity phosphatase CDC25A, M phase inducer phosphatase 1)

Cat no: C2560-01P

Supplier: United States Biological
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Cyclin-dependent kinases are positively regulated by association with cyclins and negatively regulated by binding to inhibitory subunits. The activity of cyclin-dependent kinases is also regulated by the phosphorylation status, which is controlled by the antagonistic action of wee1 kinase and CDC25 phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B, and CDC25C. These three genes function at different phases of the cell cycle. Whereas CDC25A and CDC25B are expressed throughout the cell cycle, with peak expression in G1 for CDC25A and in both G1-S-phase and G2 for CDC25B, CDC25C is predominantly expressed in G2. Applications: Suitable for use in Western Blot andI Immunoprecipitation. Other applications not tested. Recommended Dilution: Immunoprecipitation: 1-4 ul Western Blot: 1:1000-1:10,000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C2560-01P
Reactivities: Human
Hosts: Rabbit
Applications: Immunoprecipitation, Western Blot
Size: 100ug
Form: Supplied as a liquid in Tris-citrate/phosphate buffer, pH 7-8, containing 0.09% sodium azide
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography
Alternative names: Cell division cycle 25A isoform a, Cell division cycle 25A isoform b, Dual specificity phosphatase CDC25A, M phase inducer phosphatase 1
Additional info: Recognizes human cdc25A (exon 7)

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