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cdc25A (mixed clones) (Cell division cycle 25A isoform a, Cell division cycle 25A isoform b, Dual specificity phosphatase CDC25A, M phase inducer phosphatase 1)

Cat no: C2560-01S


Supplier: United States Biological
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Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry (frozen and paraffin-embedded sections). Other applications not tested. Recommended Dilution: Immunohistochemistry: 1:50-1:100 using ABC method. Incubate for 30 mins. at RT. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10mM citrate buffer, pH 6.0 prior to immunostaining. Optimal dilutions to be determined by the researcher. Cellular Localization: Nuclear membrane. Positive Control: Tonsil. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C2560-01S
Reactivities: Human, Rat
Hosts: Mouse
Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
Size: 500ul
Form: Supplied as a liquid in PBS containing sodium azide
P type: Mab
Isotype: IgG2a
Purity: Purified
Alternative names: Cell division cycle 25A isoform a, Cell division cycle 25A isoform b, Dual specificity phosphatase CDC25A, M phase inducer phosphatase 1
References: 1. eisser C et al. Subcellular localisation of Cdc25A determines cell fate. Cell Death Differ 11:134 (2004). 2. Draetta et al J Biochem Biophys Acta 1332:53 (1997). 3. Gabrielli et al Oncogene 15:749 (1997).
Additional info: Recognizes human and rat cdc25A (~65kD)

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