CDC25A belongs to the Cdc25 family phosphatases (CDC25A, CDC25B and CDC25C) which are dual-specificity phosphatases, and can dephosphorylate phosphotyrosine as well as phosphothreonine residues, therefore activating their physiological substrates, the cyclin-dependent protein kinases (Cdks) and thus have specific roles in cell-cycle regulation. Cdc25A has a comprehensive function in the cell cycle, regulating the G1/S transition, S phase and the G2/M transition. Cdc25A inactivation can contribute to an immediate block of the cell cycle, leading to an apparently p53-independent G1 arrest, an S-phase delay or a G2 arrest. Cdc25A is an unstable protein and its degradation can be critically accelerated in the presence of unreplicated orDmaged DNA. In human cells, Cdc25A is localized to the nucleus. Accelerated Cdc25A degradation, as a result of phosphorylation by the Chk1 and/or Chk2 protein kinases, results in sustained inhibitory phosphorylation of Cdk2, leading to G1 arrest and a block in S-phase entry. It often acts as an oncogene. Two transcript variants encoding different isoforms have been found for this gene.
Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 1:500-1:1000
Immunohistochemistry (paraffin): 1:50-1:100
Optimal dilutions to be determined by the researcher.
Positive Control:
A2780 cells
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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