M-phase inducer phosphatase 3, also known as CDC25C, is a tyrosine phosphatase expressed predominantly in G2 phase. It directly dephosphorylates CDC2 and triggers entry into mitosis. M-phase inducer phosphatase 3 is also thought to suppress p53-induced growth arrest. At least 5 isoforms are produced by alternative splicing.
Applications:
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:
Western Blot: 1.0-2.0ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.