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CDC25C (M-phase Inducer Phosphatase 3, Dual Specificity Phosphatase Cdc25C)

CDC25C (M-phase Inducer Phosphatase 3, Dual Specificity Phosphatase Cdc25C)

Cat no: C2560-05M1

Supplier: United States Biological
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Cyclin-dependent kinases can be activated by CDC25, which removes inhibitory phosphates from tyrosine and threonine residues. At least three CDC25 genes (CDC25A, CDC25B, and CDC25C) have been identified in humans. Whereas CDC25A and CDC25B are expressed throughout the cell cycle, with peak expression in G1 for CDC25A and in both G1-S-phase and G2 for CDC25B, CDC25C is predominantly expressed in G2. Applications: Suitable for use in Immunoprecipitation, Western Blot and Immunofluorescence. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml for 2h at RT Immunoprecipitation: 2ug/mg protein lysate Optimal dilutions to be determined by the researcher. Positive Control: HeLa, HT29 or LS174T cells Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2560-05M1
Reactivities: Human
Hosts: Mouse
Applications: Immunofluorescence, Immunoprecipitation, Western Blot
Size: 50ug
Form: Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
P type: Mab
Purity: Purified by Protein G affinity chromatography.
Additional info: Recognizes human CDC25C. Does not react with mouse and rat.

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