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Cdc6 (CDC18L, HsCDC18, HsCDC6, p62cdc6, Cell Division Cycle 6)

Cdc6 (CDC18L, HsCDC18, HsCDC6, p62cdc6, Cell Division Cycle 6)

Cat no: C2550-05B

Supplier: United States Biological
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The Cdc6 protein is an essential component of pre-replication complexes (preRCs), which assemble at origins of DNA replication during the G1 phase of the cell cycle (1). Cdc6 participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated 2). It has been concluded that expression of Cdc6 is regulated in response to mitogenic signals though transcriptional control mechanisms involving E2F proteins, and that Cdc6 is required for initiation of DNA replication in mammalian cells (3). In vitro, Cdc6 is an excellent substrate for Cdk2, phosphorylated at three sites (Serine 54, Serine 74 and Serine 106) (4). Cellular Localization: Plasma membrane Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C2550-05B
Reactivities: Yeast/Fungi
Hosts: Mouse
Applications: Immunoprecipitation, Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, pH 7.2. No preservative added.
P type: Mab
Isotype: IgG1
Purity: Purified
Alternative names: CDC6 related protein, Cell cycle controller, Cell division control protein 6, Cell division control protein 6 homolog, Cell division cycle 18, Cell division cycle 18 homolog, Cell division cycle 6 homolog, Cell division cycle 6 protein, HsCDC 18, HsCDC 6, HsCDC18 a, HsCDC6, p62(cdc6)
References: General References: 1. Hall JR, et al. Mol Biol Cell. 18(9):3340-50, 2007. 2. Williams RS, et al. Proc Natl Acad Sci U S A. 94(1):142-7, 1997. 3. Yan Z, et al. Proc Natl Acad Sci U S A. 95(7):3603-8, 1998 4. Jiang W, et al. Proc Natl Acad Sci U S A. 96(11):6193-8, 1999. This clone has been used in: Perkins G et al. Separate SCF(CDC4) recognition elements target Cdc6 for proteolysis in S phase and mitosis. EMBO J 20:4836-45 (2001). Donovan S et al. Cdc6p-dependent loading of Mcm proteins onto pre-replicative chromatin in budding yeast. Proc Natl Acad Sci U S A 94:5611-6 (1997).
Additional info: Species Crossreactivity: Crossreacts with S. cerevisiae. Not yet tested in other species.

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