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Cdc6 (CDC18L, HsCDC18, HsCDC6, p62cdc6, Cell Division Cycle 6)

Cdc6 (CDC18L, HsCDC18, HsCDC6, p62cdc6, Cell Division Cycle 6)

Cat no: C2550-06A

Supplier: United States Biological
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The replication licensing system acts to ensure that no section of the genome is replicated more than once in a single cell cycle. The origin recognition complex (ORC) and CDC6/CDC18 are needed on chromatin before the licensing reaction can take place. During the transition from a growth-arrested to a proliferative state, transcription of mammalian CDC6 is regulated by the cell cycle transcription factor (E2F protein). Immunoblots show that minichromosome maintenance (MCM) proteins are not associated with chromatin. Competence of G1 phase nuclei to replicate in vitro coincides with maximum CDC6 accumulation and MCM protein binding to chromatin in vivo. Antibodies against CDC6 and MCM5 stain abnormal (neoplastically transformed) cells in cervical smears and sections with remarkably high specificity and sensitivity. Cellular Localization: Nuclear. Positive Control: Raji or MDA231 cells. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C2550-06A
Reactivities: Human
Hosts: Mouse
Applications: Immunoprecipitation, Western Blot
Size: 500ul
Form: 10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide
P type: Mab
Isotype: IgG1
Purity: Purified by Protein G affinity chromatography.
Alternative names: CDC6 related protein, Cell cycle controller, Cell division control protein 6, Cell division control protein 6 homolog, Cell division cycle 18, Cell division cycle 18 homolog, Cell division cycle 6 homolog, Cell division cycle 6 protein, HsCDC 18, HsCDC 6, HsCDC18 a, HsCDC6, p62(cdc6)
References: Petersen BO et al. Phosphorylation of mammalian CDC6 by cyclin A/CDK2 regulates its subcellular localization. EMBO J 18:396-410 (1999).
Additional info: Species Crossreactivity: Crossreacts with Human.

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