Cadherins are calcium dependent cell adhesion molecules. E- cadherin, also known as cdh1, is expressed during early embryogenesis in blastomeres, the anterior mesoderm during gastrulation, and developing epithelial structures. Cdh1 was detected at the pharyngula stage in the midbrain-hindbrain boundary. Morpholino knockdown of E-cadherin in embryo studies was found to reduce embryo survivability; early embryonic cdh1 MO knockdown resulted in irregular cleavage plane orientation between blastomeres. Cdh1 knockdown also resulted in inhibited epiboly cell movements and gastrulation cell movements. Prechordal plate derivatives were also absent in Cdh1 knockdowns. Co-injection with E-cadherin mRNA served to demonstrate the specific activity of E-cadherin morpholino knockdown.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
ELISA: 1:5000-1:20,000
Western Blot: 1:500-1:1000
Optimal dilutions to be determined by the researcher.
Control Peptide: C0108-40D
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Quick Search
Products 
