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Cdk1, ID (Cell Division Control Protein 2, Cell Division Cycle 2, cdc2, Dr.24379)

Cdk1, ID (Cell Division Control Protein 2, Cell Division Cycle 2, cdc2, Dr.24379)

Cat no: C2571-20M

Supplier: United States Biological
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Cell division cycle 2 (cdc2) is a cell cycle control component in the eukaryotic cell cycle. In fission yeast, cdc2 is the determinant of whether a cell enters S phase or mitosis. The zebrafish homologue of cdc2 is a catalytic subunit of the maturation promoting factor (MPF), where it is coupled with the regulatory subunit cyclin B. Treatment of fully grown zebrafish oocytes with 17,20-dihydroxy-4-pregnen-3-one (17,20-DP), a natural maturing hormone in fishes, induced the oocytes to mature via activation of MPF. However, 17,20-DP treatment of growing oocytes fails to induce maturation. Immunoblotting experiments revealed that cdc2 and cdk7 concentrations are virtually constant throughout oogenesis. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:5000-1:20,000 Western Blot: 1:500-1:1000 Optimal dilutions to be determined by the researcher. Control Peptide: C2571-20Y Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2571-20M
Reactivities: Zebrafish/Fish
Hosts: Rabbit
Applications: ELISA, Western Blot
Size: 250ul
Form: Supplied as a liquid in 40mM MOPS, pH 7.5, 0.1% BSA, 0.05% sodium azide, 50% glycerol.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: 1. Hayles, J et al (1994) Cell 78:813-822. 2. Kondo, T et al (1997) Dev. Biol. 190(1):142-152.
Additional info: Recognizes zebrafish CDK-1.

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