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Cdk2 (Cell Division Protein Kinase 2, p33 Protein Kinase)

Cdk2 (Cell Division Protein Kinase 2, p33 Protein Kinase)

Cat no: C2572-05Q

Supplier: United States Biological
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CDK2 a serine/threonine protein kinase, is a member of a highly conserved family of protein kinases that regulate the eukaryotic cell cycle. The protein kinase is homologous to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. For activity, it requires association with a cyclins which act as the positive regulatory subunit. It forms complex with cyclins A, E, D1, and D3. Cyclin E- CDK2 kinase is active in the G1 and S phases of the cell cycle and is important for the progression from G1 to S phase. The levels of Cyclin A- CDK2 are maximal at the G1/S transition and both CDK2 and Cyclin A associate with DNA in the initiation complex during replication. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:1000 Optimal dilutions to be determined by the researcher. Positive Control: A2780 cells Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2572-05Q
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: 1. Akiyama, T. et al. Cancer. Res. 57:1495-1501 (1997). 2. Bresnahan, WA. et al. J. General. Virol. 78:1993-1997 (1997).
Additional info: Recognizes human CDK2. Species Crossreactivity: mouse and rat.

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