CDK3, Cyclin E1, Active, Recombinant, Human, GST-Tag (Cyclin-dependent Kinase 3, Cell Division Protein Kinase 3, CDK3, CDKN3G1/S-specific Cyclin-E1, CCNE1, CCNE)

Serine/threonine-protein kinase that plays a critical role in the control of the eukaryotic cell cycle; involved in G0-G1 and G1-S cell cycle transitions. Interacts with CCNC/cyclin-C during interphase. Phosphorylates histone H1, ATF1, RB1 and CABLES1. ATF1 phosphorylation triggers ATF1 transactivation and transcriptional activities, and promotes cell proliferation and transformation. CDK3/cyclin-C mediated RB1 phosphorylation is required for G0-G1 transition. Promotes G1-S transition probably by contributing to the activation of E2F1, E2F2 and E2F3 in a RB1-independent manner.\n\nCyclin E1 functions as a regulator of the cell cycle, promoting the G1 to S phase transition by binding to and activating the cyclin dependent kinase CDK2. The abundance of cyclin E1 fluctuates during a normal cell cycle and is controlled at the level of gene transcription and by ubiquitin dependent proteolysis. Non-complexed cyclin E1 is degraded by the Cul3 pathway, however when cyclin E1 is bound to CDK2 its ubiqutination is dependent on a positive phosphorylation status, which enables its recognition by SCFFbw7. The impact of cyclin E1 quantities on cell cycle regulation is reflected in the elevated levels expressed by many types of human tumors.\n\nSource:\nRecombinant corresponding to complex of N-terminal GST-tagged full length human CDK3 and Cyclin E1 co-expressed in a Baculovirus-infected Sf9 cell expression system.\n\nMolecular Weight:\nCDK3: ~61kD; Cyclin E1: ~74kD\n\nSpecific Activity:\n~30pmole/min/mg\n\nApplications:\nSuitable for use in three-dimensional structure studies. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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