IKKalpha, an IkappaB -type protein kinase, is a member of the multimeric IkappaB kinase complex. This complex, often consisting of IKKalpha/IKKbeta heterodimers plus regulatory IKKgamma subunits, phosphorylates IkappaB proteins, marking them for destruction and thereby allowing activation of NFkappaB and downstream nuclear genes. The constitutively expressed IKKalpha protein contains a N-terminal kinase domain, a central leucine zipper motif, and a C-terminal helix-loop-helix motif. In knockout studies, IKKalpha (-/-) mice died perinatally and displayed a range of developmental defects; thus, IKKalpha is believed to function in development, particularly in development of skin keratinocyte, cornea, and conjunctiva.
Applications:
Suitable for use in Western Blot, ELISA, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
ELISA: 1:10,000-1:50,000
Western Blot: 1:500-1:3000
Immunohistochemistry (Formalin fixed paraffin embedded): 1:500
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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