Cyclin-dependent kinase 9, CDK9, is a member of the cell division cycle 2 (CDC2)-like family of kinases with roles in different cellular processes such as signal transduction, basal transcription, HIV-Tat- and MyoD-mediated transcription and differentiation. The cyclin-dependent kinase pair CDK9/cyclin T1 complex, also called positive transcription elongation factor b (P-TEFb), is very important in differentiation of several cell types, possibly including lymphoid cells (1-2). The association of CDK9 with the HIV-Tat protein suggests a possible involvement in AIDS, and its responsiblility for the kinase activity associated with the TAK and P-TEFb complexes suggests its involvement in the transcription process (3).
Applications:
Suitable for use in Western Blot, Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 1:2000-1:5000
Immunohistochemistry: 1:100-1:250
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.