CDKN1A, Recombinant, Human (Cyclin-dependent Kinase Inhibitor 1, CDK-interacting Protein 1, Melanoma Differentiation-associated Protein 6, MDA-6, p21, CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WAF1)

p21/WAF1/Cip1/Sdi1/Pic1 is a tumor suppressor protein. Expression of p21/WAF1 is induced by wild type, but not mutant, p53 suppressor protein. The p21/WAF1 protein binds to cyclin/CDK complexes and inhibits their kinase activity thereby stopping cell cycle progression. Meanwhile, the p21/WAF1 protein also binds to PCNA (proliferating cell nuclear antigen) and blocks DNA replication but not the DNA repair process. Evidently, inactivation of p53 disrupts the p53-dependent expression of p21/WAF1. p21/WAF1is a tumor suppressor involved in the pathogenesis of a variety of malignancies.\n\nSource: \nRecombinant corresponding to human CDKN1A, expressed in E.coli.\n\nMolecular Weight:\n~47kD\n\nEndotoxin: ~0.1ng/ug. Diners and Aggregates: <1% as determined by coomassie blue SDS-PAGE gel analysis.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

136881

Size

2ug

Reactivities

Hum

Form

Supplied as a liquid in 50mM Tris-Acetate, pH7.5, 1mM EDTA, 20% gycerol. No preservative added.

Purity

~90% (HPLC and SDS-PAGE)

References

1. P21/WAF1 is an independent survival prognostic factor for patients with hepatocellular carcinoma after resection. Liver Int 2007 Aug;27(6):772-81. 2. Immunoreactivity of p53, Mdm2, p21(WAF1/CIP1) Bcl-2, and Bax in soft tissue sarcomas: correlation with histologic grade. Appl Immunohistochem Mol Morphol 2007 Mar;15(1):64-9. 3. Sp1 and Smad3 are required for high glucose-induced p21(WAF1) gene transcription in LLC-PK(1) cells. J Cell Biochem 2007 May 21. 4. p21(Waf1/Cip1/Sdi1) mediates retinoblastoma protein degradation. Oncogene 2007 May 7. 5. Expression of p21(WAF1) is related to acetylation of histone H3 in total chromatin in human colorectal cancer. World J Gastroenterol 2007 Apr 21;13(15):2209-13. 6. Expression of the HER family mRNA in breast cancer tissue and association with cell cycle inhibitors p21(waf1) and p27(kip1). Anticancer Res 2007 Mar-Apr;27(2):913-20.