CEBP alpha, aa270-358, bZIP Region, Recombinant, Human, His-tag (CCAAT Enhancer Binding Protein, alpha)

CCAAT/enhancer binding protein (C/EBP) alpha is a family of transcription factors that all contain a highly conserved, basic-leucine zipper domain at the C-terminus that is involved in dimerization and DNA binding. C/EBP family of transcription factors regulates viral and cellular CCAAT/enhancer element-mediated transcription. C/EBP family consist of several related proteins, C/EBP alpha, beta, gamma, delta, that form homodimers and that form heterodimers with each other. C/EBP proteins contain the bZIP region, which is characterized by two motifs in the C-terminal half of the protein; a basic region involved in DNA binding and a leucine zipper motif involved in dimerization. C/EBPs differ significantly in their physiological functions and in their downstream target genes. For example, mice lacking C/EBP alpha die shortly after birth due to severe hypoglycemia and the absence of glycogen storage in liver, whereas knockout of C/EBP beta causes defects in female reproduction.\n\nMolecular Weight: \n14.5kD (126 amino acids)\n\nSequence:\nMRGSHHHHHH/ GMASMTGGQQ/ MGRDLYDDDD/ KDRWGSMGAG/ KAKKSVDKNS/ NEYRVRRERN/ NIAVRKSRDK/ AKQRNVETQQ/ KVLELTSDND/ RLRKRVEQLS/ RELDTLRGIF/ RQLPESSLVK/ AMGNCA\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2589-88

Size

250ug

Form

Supplied as a liquid in 20mM Tris-HCl, pH 7.5, 0.1M sodium chloride, 5mM beta-Mercaptoethanol.

Purity

(same/more than) 95% by SDS PAGE; Purified by ion-exchange chromatography and FPLC gel-filtration chromatography.

References

1. Antoson, P. & Xanthopouos, K.G., Biochem. Biophys. Res. Commun. 215(1): 106-113 (1995). 2. Subramanian, L., et al., J. Biol. Chem. 278(11): 9134-9141 (2003). 3. Gombart, A.F., et al., Blood 99(4): 1332-1340 (2002).