CEBP alpha, aa270-358, bZIP Region, Recombinant, Human, His-tag (CCAAT Enhancer Binding Protein, alpha)

CCAAT/enhancer binding protein(C/EBP) alpha is a family of transcription factors that all contain a highly conserved, basic-leucine zipper domain at the C-terminus that is involved in dimerization and DNA binding. C/EBP family of transcription factors regulates viral and cellular CCAAT/enhancer element-mediated transcription. C/EBP family consist of several related proteins, C/EBP alpha,beta,gamma,delta, that form homodimers and that form heterodimers with each other. C/EBP proteins contain the bZIP region, which is characterized by two motifs in the C-terminal half of the protein; a basic region involved in DNA binding and a leucine zipper motif involved in dimerization. C/EBPs differ significantly in their physiological functions and in their downstream target genes. For example, mice lacking C/EBPa die shortly after birth due to severe hypoglycemia and the absence of glycogen storage in liver, whereas knockout of C/EBPbeta causes defects in female reproduction\n\nRecombinant Human CEBP-alpha (aa270-358) was purified by ion-exchange chromatography and FPLC gel-filtration chromatography. Recombinant Human CEBP-a His-Tag fusion proein produced in E.Coli is a single,non-glycosylated polypeptide chain containing amino acids 126 and having a molecular mass of 14.5kD.\n\nAA Sequence: \nMRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMGAG KAKKSVDKNS NEYRVRRERN NIAVRKSRDK AKQRNVETQQ KVLELTSDND RLRKRVEQLS RELDTLRGIF RQLPESSLVKAMGNCA\n\nStability: \nStore at 4 degrees C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degrees C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

C2589-88A

Size

10ug

Form

Supplied as a liquid in 20mM Tris-HCl, pH 7.5, 0.1M sodium chloride and 5mM beta-Mercaptoethanol.

Purity

(same/more than) 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.

References

1. Arch Physiol Biochem (2006) Feb;112(1):3-12. 2. Endocrinology (2006) Jun 1. 3. Am J Physiol Lung Cell Mol Physiol (2006) May 12. 4. Exp Physiol (2006) Jul;91(4):747-53. 5. Blood (2006) May 2. 6. Blood (2006) Apr 27.