CEBP gamma, aa39-147, DNA Binding Domain, Recombinant, Human, His-tag (CCAAT Enhancer Binding Protein, gamma)

CCAAT/enhancer binding protein (C/EBP) gamma is a family of transcription factors all contain a highly conserved, basic-leucine zipper domain at the C-terminus that is involved in dimerization and DNA binding. C/EBP family of transcription factors regulates viral and cellular CCAAT/enhancer element-mediated transcription. C/EBP family consist of several related proteins, C/EBP alpha, beta, gamma, delta, that form homodimers and/or form heterodimers with each other. C/EBP proteins contain the bZIP region, which is characterized by two motifs in the C-terminal half of the protein; a basic region involved in DNA binding and a leucine zipper motif involved in dimerization. C/EBP gamma may cooperate with Fos to bind PRE-enhancer elements. The DNA binding domain of CEBP gamma (amino acid residues, 39-147) was produced in E .coli and purified by using conventional chromatography techniques.\n\nMolecular Weight: 16.5kD (146 amino acids)\n\nSequence:\nMRGSHHHHHH/ GMASMTGGQQ/ MGRDLYDDDD/ KDRWGSMPGG/ GGKAVAPSKQ/ SKKSSPMDRN/ SDEYRQRRER/ NNMAVKKSRL/ KSKQKAQDTL/ QRVNQLKEEN/ ERLEAKIKLL/ TKELSVLKDL/ FLEHAHNLAD/ NVQSISTENT/ TADGDN\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2589-94

Size

250ug

Form

Supplied as a liquid in 20mM Tris-HCl, pH 7.5, 0.1M sodium chloride, 5mM beta-Mercaptoethanol.

Purity

Purified by conventional chromatography techniques ((same/more than) 95% by SDS PAGE).

References

1. Hattori, T., et al., Oncogene 22(9): 1273-1280 (2003). 2. Davydov, I., et al., Gene 161(2): 271-275 (1995). 3. Williams, S.C., et al., Genes Dev. 5(9): 1553-1567 (1991).