CEBP gamma, aa39-147, DNA Binding Domain, Recombinant, Human, His-tag (CCAAT Enhancer Binding Protein, gamma)

CCAAT/enhancer binding protein(C/EBP) gamma is a family of transcription factors all contain a highly conserved, basic-leucine zipper domain at the C-terminus that is involved in dimerization and DNA binding. C/EBP family of transcription factors regulates viral and cellular CCAAT/enhancer element-mediated transcription. C/EBP family consist of several related proteins, C/EBP alpha,beta,gamma,delta, that form homodimers and/or form heterodimers with each other. C/EBP proteins contain the bZIP region, which is characterized by two motifs in the C-terminal half of the protein; a basic region involved in DNA binding and a leucine zipper motif involved in dimerization. C/EBP g may cooperate with Fos to bind PRE- enhancer elements.\n\nDescription: \nRecombinant Human CEBP-gamma His-Tag fusion proein produced in E.Coli is a single,non-glycosylated polypeptide chain containing amino acids 146 and having a molecular mass of 16.5kD.\n\nThe DNA binding domain of CEBP-g (amino acid residues, 39-147) was purified by using conventional chromatography techniques\n\nSequence: \nMRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMPGG GGKAVAPSKQ SKKSSPMDRN SDEYRQRRER NNMAVKKSRL KSKQKAQDTL QRVNQLKEEN ERLEAKIKLL TKELSVLKDL FLEHAHNLAD NVQSISTENT TADGDN\n\nStability: \nStore at 4 degrees C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degrees C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

C2589-94A

Size

10ug

Form

Supplied as a liquid in 20mM Tris-HCl, pH 7.5, 0.1M sodium chloride and 5mM beta-Mercaptoethanol.

Purity

(same/more than) 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.

References

1. C/EBP gamma has a stimulatory role on the IL-6 and IL-8 promoters.\n\nJ Biol Chem 2002 Oct 11;277(41):38827-37\n\n2. Ig/EBP (C/EBP gamma) is a transdominant negative inhibitor of C/EBP family transcriptional activators.\n\nNucleic Acids Res 1995 Nov 11;23(21):4371-7\n\n3. Cloning of the cDNA encoding human C/EBP gamma, a protein binding to the PRE-I enhancer element of the human interleukin-4 promoter.\n\nGene 1995 Aug 19;161(2):271-5\n\n4. Molecular cloning of two C/EBP-related proteins that bind to the promoter and the enhancer of the alpha 1-fetoprotein gene. Further analysis of C/EBP beta and C/EBP gamma.\n\nNucleic Acids Res 1992 Jun 25;20(12):3091-8