Cell Staining, Live-Dead BioAssay(TM) Kit
Distinguishing between live and dead cells is very important for investigation of growth control and cell death. The Cell Staining, Live-Dead BioAssay(TM) Kit provides ready-to-use staining reagents to conveniently discriminate between live and dead cells. The kit utilizes Cyto-dye, a cell-permeable green fluorescent dye (Ex/Em = 488/518 nm), to stain live cells. Dead cells can be easily stained by propidium iodide (PI), a cell non-permeable red fluorescent dye (Ex/Em = 488/615). Stained live and dead cells can be visualized by fluorescence microscopy using a band-pass filter (detects FITC and rhodamine). Cyto-Dye has also been used to discriminate between apoptotic and non-apoptotic cells.
Kit Components:
C2608A: CYTO-Dye, 1mM 50ul
C2608B: PI, 2.5mg/ml 50ul
C2608C: Staining buffer 50ml*
Storage and Stability:
Store C2608A and C2608B at -20 degrees C. Stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Store C2068C at 4 degrees C. Stable for 12 months.