Cells, PBMC, CD14+ Monocytes, Negative Selection, BioPrimary(TM)

BioPrimary(TM) Cells are derived using technology including advanced protocols and adjuvants that ehance the viability of our cells to over >90% total post-thaw viability. CD+ Monocytes are also tested for functionality by observing their differentiation to CD86+ Dendritic Cells.\n\nMonocytes have two main functions in the immune system: (1) replenish resident macrophages and dendritic cells under normal states, and (2) in response to inflammation signals, monocytes can move quickly (approx. 8-12 hours) to sites of infection in the tissues and divide/differentiate into macrophages and dendritic cells to elicit an immune response. Half of them are stored in the spleen. Monocytes are usually identified in stained smears by their large bilobate nucleus. Monocytes are produced by the bone marrow from hematopoietic stem cell precursors called monoblasts. Monocytes circulate in the bloodstream for about one to three days and then typically move into tissues throughout the body. They constitute between three to eight percent of the leukocytes in the blood.\n\nMonocytes can perform phagocytosis using intermediary (opsonising) proteins such as antibodies or complement that coat the pathogen, as well as by binding to the microbe directly via pattern-recognition receptors that recognize pathogens. Monocytes are also capable of killing infected host cells via antibody, termed antibody-mediated cellular cytotoxicity.\n\nRole in Disease:\nMonocytosis\nMonocytosis is the state of excess monocytes in the peripheral blood. It may be indicative of various disease states. Examples of processes that can increase a monocyte count include:\nchronic inflammation\nstress response\nhyperadrenocorticism\nimmune-mediated disease\ninfectious mononucleosis\npyogranulomatous disease\nnecrosis\nred cell regeneration\nViral Fever\nMonocytopenia\n\nAll products are shipped on dry ice with: 1) Certificate of Analysis (with viability and purity results), and 2) Thawing instructions.\n\nViability:\n~90%\n\nPost Thaw Viability:\n~90%\n\nMycoplasma Testing:\nNegative\n\nSterility Testing: \nNegative\n\nInfectious Disease Screens:\nHIV, type1: Negative\nHIV, type 2: Negative\nHep B virus (surface antigen and core): Negative\nHep C virus: Negative\nHuman T-cell lymphotropic virus, type 1: Negative\nHuman T-cell lymphotropic virus, type 2: Negative\nCytoMegalo Virus (CMV): Negative\n\nStorage and Stability: \nIt is recommended that cells are used as soon as possible following receipt to ensure optimal viability. Cells may be stored at -80 C for up to one month (minimize storage length at -80 C in order to maximize viability).

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