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CENP-A (Centromere Protein A, Histone H3-like Centromeric Protein A, Centromere Autoantigen A)

CENP-A (Centromere Protein A, Histone H3-like Centromeric Protein A, Centromere Autoantigen A)

Cat no: C2615-10A

Supplier: United States Biological
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Centromere protein A (CENP-A) is a 17kD centromere-specific histone variant with 62% amino acids homology to the C-terminal of histone H3. Localized in the centromere, it plays a central role in the centromere-specific chromatin formation. The depletion of histone H3 at the CENP-A binding domain suggests CENP-A to be a possible replacement for histone H3 in the packaging process of a-satellite DNA into primary chromatin structure. CENP-A is essential in the formation of specialized nucleosomes at the centromere, implicating CENP-A as a centromere-specific epigenetic marker. Applications: Suitable for use in Flow Cytometry, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:80 Western Blot: 1:5000 Immunohistochemistry: 1:250-1:500 Immunocytochemistry: 1:250-1:500 Optimal dilutions to be determined by the researcher. Positive Control: HeLa Whole Cell lysate. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C2615-10A
Reactivities: Human, Rat
Hosts: Rabbit
Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blot
Size: 100ul
Form: Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.
P type: Mab
Isotype: IgG
Purity: Supernatant
References: 1. Durand-Dubief M, Ekwall K. (2008). Bioessays. 30(6):526-9 2. Black BE, Bassett EA. (2008). Curr Opin Cell Biol. 20(1):91-100 3. Palmer DK, et al. (1987). J Cell Biol. 104(4):805-15.
Additional info: Recognizes the C-terminal region of human CENP-A. Species Crossreactivity: rat. Does not react with mouse.

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