CENP-A, Recombinant, Human (Centromere Protein A)

Centromere proteins are a group of proteins which form and/or mediate the function of centromeres, the central structures of chromosomes to which spindle fibers/microtubuli attach and pull the chromosomes apart in cell division. Currently, 9 centromere proteins are known and designated CENP-A to CENP-I. Most of the centromere proteins are targets of autoantibodies, the anti-centromere antibodies. CENP-A is another important centromeric autoantigen in addition to CENP-B: it has a molecular weight of approx. 20 kD and is incorporated into centromeric chromatin due to its histone-like properties. CENP-A antibodies are an important marker for correct diagnosis of Scleroderma / CREST syndrome in CENP-B-autoantibody negative patients.\n\nDescription: \nRecombinant Human CENP-A produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 17,015 dalton. Recombinant CENP-A is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.\n\nImmunological Functions:\n1. Binds IgG-type human auto-antibodies.\n2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera).\n\nApplications: \nWestern-Blot with monoclonal anti-hexa-His-tag antibody & Scleroderma patient sera.\n\nCoating Concentration:\n0.15-0.4ug/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.\n\nStability: \nStore at 4 degrees C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degrees C for longer periods of time. Please avoid freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

C2615-05B

Size

5ug

Form

Supplied as a liquid in 16mM HEPES buffer, pH 8.0, 250mM sodium chloride and 6M urea.

Purity

(same/more than) 90% (SDS-PAGE)

References

1. Comprehensive analysis of the ICEN (Interphase Centromere Complex) components enriched in the CENP-A chromatin of human cells.\n\nGenes Cells 2006 Jun;11(6):673-84\n\n2. The CENP-H-I complex is required for the efficient incorporation of newly synthesized CENP-A into centromeres.\n\nNat Cell Biol 2006 May;8(5):446-57\n\n3. The human CENP-A centromeric nucleosome-associated complex.\n\nNat Cell Biol 2006 May;8(5):458-69\n\n4. Differential role of CENP-A in the segregation of holocentric C. elegans chromosomes during meiosis and mitosis.\n\nNat Cell Biol 2005 Dec;7(12):1248-55\n\n5. Xenopus CENP-A assembly into chromatin requires base excision repair proteins.\n\nDNA Repair (Amst) 2005 Jul 12;4(7):760-72\n\n6. The CHD remodeling factor Hrp1 stimulates CENP-A loading to centromeres.\n\nNucleic Acids Res 2005 May 20;33(9):2868-79