CENP-B, Recombinant, Human (Centromere Protein B)

Centromere proteins are a group of proteins which form and/or mediate the function of centromeres, the central structures of chromosomes to which spindle fibers/microtubuli attach and pull the chromosomes apart in cell division. Currently, 9 centromere proteins are known and designated CENP-A to CENP-I. Most of the centromere proteins are targets of autoantibodies, the anti-centromere antibodies. CENP-B is the most important of the centromeric autoantigens: it has a molecular weight of approx. 66kD and plays an important role in the formation of the centromeric chromatin. CENP-B antibodies are present in the sera of up to 80% of patients with CREST syndrome.\n\nDescription: \nRecombinant Human CENP-B produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 65,940 dalton. Recombinant CENP-B is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.\n\nImmunological Functions:\n1. Binds IgG-type human auto-antibodies.\n2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera).\n\nApplications: \nSuitable for use in ELISA, Western Blot, for biotinylation and iodination.. Other applications not tested. \n\nRecmmended Dilution:\nELISA: 0.15-0.375ug/ml (depending on the type of ELISA plate and coating buffer).\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2615-12H

Size

5ug

Form

Supplied as a liquid in 20mM HEPES buffer, pH 7.6, 250mM sodium chloride and 20% glycerol.

Purity

(same/more than) 90% (SDS-PAGE)

References

1. Anti-endothelial cell antibodies from patients with limited cutaneous systemic sclerosis bind to centromeric protein B (CENP-B). Clin Immunol 2006 Mar 28; 2. Molecular characterization of the first satellite DNA with CENP-B and CDEIII motifs in the bat Pipistrellus kuhli. FEBS Lett 2005 Apr 25;579(11):2519-27 3. CpG methylation of the CENP-B box reduces human CENP-B binding. FEBS J 2005 Jan; 272(1):282-9 4. The role of CENP-B and alpha-satellite DNA: de novo assembly and epigenetic maintenance of human centromeres. Chromosome Res 2004;12(6):543-56 5. CENP-B is a conserved gene among vegetal species. Genet Mol Res 2002 Sep 30;1(3):241-5 6. CENP-B interacts with CENP-C domains containing Mif2 regions responsible for centromere localization. J Biol Chem 2004 Feb 13; 279(7):5934-46