The B-Bridge Ceruloplasmin Activity Kit is designed to quantitatively measure ceruloplasmin activity in urine samples. A human ceruloplasmin standard is provided to generate a standard curve for the assay. Samples are diluted in the provided Assay Buffer and added to the wells of a half area clear plate. The reconstituted Ceruloplasmin Substrate is added and the plate is incubated at 30Degree C for 60 minutes. The ceruloplasmin in the samples reacts with the substrate to produce a colored product. The optical density is read at 560 nm. Increasing levels of ceruloplasmin in the samples causes an increase in the fuschia (pinkpurple) product. The results are expressed in terms of units of ceruloplasmin activity per mL.
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