Human Ceruloplasmin (CER) is officially known as ferroxidase or iron(II):oxygen oxidoreductase. Human Ceruloplasmin (CER) is an enzyme synthesized in the liver containing 8 atoms of copper in its structure. Although Human CERULOPLASMIN (CER) is often considered a copper transport protein, this is not its primary function, just as human hemoglobin is not a carrier of iron, although it contains iron. The main carrier for copper in the plasma of humans is albumin. Human Ceruloplasmin enzyme (ferroxidase) catalyzes the oxidation of ferrous iron (Fe2+) to ferric iron (Fe3+), therefore assisting in Human Ceruloplasmin enzyme transport in the plasma in association with transferrin, which can only carry iron in the ferric state.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
ELISA: 1:200-1:500
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies:
I1906 IgY, Chicken Pab Rb x Ch
I1906-10 IgY, Chicken (HRP) Rb x Ch
I1906-12 IgY, Chicken (FITC) Rb x Ch
I1906-14 IgY, Chicken (AP) Rb x Ch
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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