Home  >  Products  >  CETN1, Recombinant, Human, aa1-172, His-Tag (Centrin-1, Caltractin Isoform 2, CEN1, CETN)

CETN1, Recombinant, Human, aa1-172, His-Tag (Centrin-1, Caltractin Isoform 2, CEN1, CETN)

Cat no: 045414


Supplier: United States Biological
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Centrin, also known as CETN1, is an EF-hand type Ca2+-binding protein. It is localized to the centrosome of interphase cells, and redistributes to the region of the spindle poles during mitosis, reflecting the dynamic behavior of the centrosome during the cell cycle. CETN1 plays an important role in the determination of centrosome position and segregation, and in the process of microtubule severing. Source: Recombinant corresponding to aa1-172 from human CETN1, fused to His-tag at N-terminus, expressed in E.coli. Molecular Weight: ~22.1kD (196aa) confirmed by MALDI-TOF AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMASGFK KPSAASTGQK RKVAPKPELT EDQKQEVREA FDLFDVDGSG TIDAKELKVA MRALGFEPRK EEMKKMISEV DREGTGKISF NDFLAVMTQK MSEKDTKEEI LKAFRLFDDD ETGKISFKNL KRVANELGEN LTDEELQEMI DEADRDGDGE VNEEEFLRIM KKTSLY Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: 045414
Size: 50ug
Form: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 1mM DTT, 0.1M sodium chloride, 10% glycerol.
Purity: ~90% (SDS-PAGE)
References: 1. LeDizet M., et al. (1998) Am J Physiol. 275:L1145-1156. 2. Araki M., et al. (2001) J Biol Chem. 276: 18665-18672.

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