CFHR4 (Complement Factor H-related protein 4; also FHR4B) is a 45kD glycoprotein member of the factor H-related protein family. It is synthesized by hepatocytes and circulates in plasma. FHR4(B) binds to C3b and C3d. It also binds to native (pentameric) C-reactive protein (pCRP), and is suggested to promote pCRP binding to necrotic cells surfaces, thus promoting cell clearance. Mature human FHR4B is 312aa in length. It contains five Sushi/SCR domains, the first of which (aa23-85) is associated with pCRP binding. There is a circulating, 86kD alternative splice form termed FHR4A that shows an insertion of 247aa after Asp92. This is considered the dominant isoform, and appears to show the same activity as FHR4B. Over (aa41 -331, human FHR4B shares 61% aa identity with a related molecule found in rat.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
Direct ELISA: 0.5-1ug/ml
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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