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CFL1 (Cofilin-1, Cofilin, Non-muscle Isoform, 18kD Phosphoprotein, p18, CFL)

Cat no: 124903

CFL1 (Cofilin-1, Cofilin, Non-muscle Isoform, 18kD Phosphoprotein, p18, CFL)

Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner.\n\nApplications:\nSuitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence: 10ug/ml\nImmunohistochemistry (Formalin fixed paraffin embedded): 1.5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nAA Sequence:\nMASGVAVSDGVIKVFNDMKVRKSSTPEEVKKRKKAVLFCLSEDKKNIILEEGKEILVGDVGQTVDDPYATFAKMLPDKDCRYALYDATYETKESKKEDLVFIFWAPESAPLKSKMIYASSKDAIKKKLTGIKHELQANCYEGVKDRCTLAEKLGGSAVISLEGKPL\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

124903

Size

100ug

Applications

ELISA, IF, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, pH 7.2.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2a,k

References

1. Identification of phosphorylated serine-15 and -82 residues of HSPB1 in 5-fluorouracil- resistant colorectal cancer cells by proteomics. Sakai A, Otani M, Miyamoto A, Yoshida H, Furuya E, Tanigawa N.Journal of Proteomics (2011), doi: 10.1016/j.jprot.2011.09.023. 2. Overexpression of cofilin 1 can predict progression-free survival in patients with epithelial ovarian cancer receiving standard therapy. Nishimura S, Tsuda H, Kataoka F, Arao T, Nomura H, Chiyoda T, Susumu N, Nishio K, Aoki D.Hum Pathol. 2011 Jan 13. [Epub ahead of print]

Additional Info

Recognizes human CFL1. Species Crossreactivity: mouse.

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