Chapsyn 110 (PSD93, Discs Large Homolog 2, DLG2, Channel-associated Protein of Synapses)

Post Synaptic Density 93 (PSD 93), also known as chapsyn-110, is one of a family of plasma membrane-associated proteins found in synaptic junctions. PSD 93 is unique among family members in its expression in Purkinje neuron cell bodies and dendrites. PSD 93 has three ~90 amino acid repeats called PDZ domains, a single interior SH3 domain, and a carboxyl-terminal guanylate kinase homology (GuK) domain that is enzymatically inactive. It is hypothesized that PDZ-domain interactions play a role in receptor and channel clustering which contributes to neuronal plasticity.PSD 93 is believed to participate in the clustering of certain proteins, including N-methyl-D-aspartate (NMDA) receptors and shaker-type potassium channels at the synaptic membrane. There are two principal modes of interaction between PSD 93 and other proteins. NMDA receptors and shaker-type potassium channels both share C-terminal sequence homology consisting of a threonine/serine-X-valine-COOH (T/SXV) motif. Other neuronal proteins that share this motif (beta 1 adrenergic receptor, some serotonin receptors, some sodium channel subunits, and additional potassium channel subunits) may interact with PSD 93 by binding to its PDZ domains. Neuronal nitric oxide synthase (nNOS), which lacks the T/SXV motif but which has its own PDZ domain, has been shown to associate with PSD 93 in vitro through a pseudo-homotypic PDZ-PDZ interaction.\n\nApplications: \nSuitable for use in Western Blot and Immunohistochemistry (Fr). Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C3550-04

Size

100ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a liquid in PBS, 1 mg/ml BSA, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

Gomperts SN Clustering membrane proteins: It's all coming together with the PSD-95/SAP90 protein family. Cell 84:659-62 (1996). <PUBMED:8625403>Kim E et al. Heteromultimerization and NMDA receptor-clustering activity of Chapsyn-110, a member of the PSD-95 family of proteins. Neuron 17:103-13 (1996). <PUBMED:8755482>Brenman JE et al. Cloning and characterization of postsynaptic density 93, a nitric oxide synthase interacting protein. J Neurosci 16:7407-15 (1996). <PUBMED:8922396>

Additional Info

Detects Post Synaptic Density 93 (PSD 93) from rat tissues. Does not detect other synapse-associated protein family members. Species Crossreactivity: Rat. Expected to crossreact with mouse (100% identity with immunogen) and human (100% identity with immunogen) due to sequence homology.

Alternative Names

PSD93, Discs Large Homolog 2, DLG2, Channel-associated Protein of Synapses 110kD