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Chemokine (C-C Motif) Ligand 5 (CCL5) ELISA Kit

Chemokine (C-C Motif) Ligand 5 (CCL5) ELISA Kit

Cat no: ABIN414328

Supplier: Antibodies-online
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Chemokine (C-C Motif) Ligand 5 (CCL5) ELISA Kit: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm
Catalogue number: ABIN414328
Size: 96 tests
Accession: P97272
Gene: 100135495
Antigen: RANTES
Sensitivity: The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
Assay range: 0.156-10 ng/mL
Sample type: Serum,Plasma,Tissue Homogenate,Cell Culture Supernatant,Biological Fluids
Entrez gene: 100135495

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