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CHIP (Carboxy Terminus of Hsp70p-interacting Protein, STUB1, STIP1 Homology and U-Box Containing Protein 1, HSPABP2, NY-CO-7, SDCCAG7, Serologically Defined Colon Cancer Antigen 7, Heat Shock Protein A Binding Protein 2 (C-terminus))

Cat no: C4125-03C

CHIP (Carboxy Terminus of Hsp70p-interacting Protein, STUB1, STIP1 Homology and U-Box Containing Protein 1, HSPABP2, NY-CO-7, SDCCAG7, Serologically Defined Colon Cancer Antigen 7, Heat Shock Protein A Binding Protein 2 (C-terminus))

STUB1 (STIP1 homology and U-box-containing protein 1, also known as C-terminus of Hsc70-interacting protein, CHIP) is a 35kD ubiquitin ligase that participates in intracellular protein folding/refolding and degradation. It is highly expressed in striated muscle. STUB1 may complex with the molecular chaperones Hsp70 and Hsp90, or act by itself. In either event, it regulates protein stability and ubiquitination. Human STUB1 is 303 amino acids (aa) in length. It contains one TPR domain (aa 27-127) plus a C-terminal U-Box (aa 226-299). Its TRP domain interacts with heat shock proteins, while the U-Box participates in ubiquitination. There is one isoform that shows an alternate start site at Met73. Human and mouse STUB1 are 97% aa identical.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1.0ug/ml.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C4125-03C

Size

100ug

Applications

WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 100ul sterile 40-50% glycerol, PBS 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes human STUB1/CHIP.

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