Chitinase, Trichoderma viride (Chitodextrinase, N-acetyl-b-glucosaminidase)

The chitinolytic enzymes from T. viride are a mixture of extracellular chitinolytic enzymes, which exhibit exo and endochitinase activities including N-acetyl-b-glucosaminidase and chitobiosidase.\n\nChitinases are digestive enzymes that break down glycosidic bonds in chitin. Because chitin composes the cell walls of fungi and exoskeletal elements of some animals (including worms and arthropods), chitinases are generally found in organisms that either need to reshape their own chitin or to dissolve and digest the chitin of fungi or animals.\n\nSolubility:\nSoluble in phosphate buffer, pH 6.1, (1mg/ml), yielding a clear to faint hazy, tan solution\n\nChitinase Activity: (same/more than)600 units/g solid \nChitobiosidase Activity: \nAs reported\nNagase Activity:\nAs reported\nOptimum pH: 7.8~8.6 \n\npH Stability (30 degrees C, 1 hour): 6.6~9.0 \n\nTemperature Stability: 45 degrees C (10 min.)\nChitinase Unit Definition: \nOne unit will liberate 1mg of N-acetyl-D-glucosamine from chitin per hour at pH 6.0 at 25 degrees C in a 2 hour assay. \n\nChitobiosidase Unit Definition: \nN-Acetyl-b-glucosaminidase\n[Chitobiosidase]: One unit will release 1.0 mg of 4-nitrophenol from 4-nitrophenyl N-Acetyl-b-Dglucosaminide [4-nitrophenyl N,N'-diacetyl -b--Dchitobioside] per minute at pH 6.1 at 40 degrees C.\n\nAssay:\nReaction mixture is composed of 30mg of N-acetyl chitosan, 2ml of 0.1M Mcllvaine buffer (pH 6.8) and 1ml of enzyme solution. After incubation at 37 degrees C for 60 minutes, the reaction of the enzyme is terminated by heating at 100 degrees C for 5 minutes and the amount of reducing sugar is determined by the method of Schales and Schales, using N-acetyl glucosamine as a standard.\n\nStorage & Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute with sterile PBS. Store at -20 degrees C. Reconstituted product is stable for 5 weeks at 4 degrees C. For maximum recovery of product, centrifuge the original vial prior to removing the cap

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SPECIFICATIONS

Catalog Number

C4154-20A

Size

25U

Form

Supplied as a lyophilized powder.

References

1. Ohtakara, A., Izume, M. and Mitsutomi, M.: Agric, Biol. Chem., 52, 3181 (1988) 2. Hirano, S. and Yamaguchi, R.: Biopolymers, 15, 1685 (1976) 3. Schales, O. and Schales, S.: Arch. Biochem., 8, 285 (1945)

Alternative Names

EC=3.2.1.14; Chitodextrinase; N-acetyl-b-glucosaminidase