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Chk 1 (Checkpoint Kinase 1, CHEK1, CHK1, Serine/threonine-protein Kinase Chk1)

Cat no: 030095

Chk 1 (Checkpoint Kinase 1, CHEK1, CHK1, Serine/threonine-protein Kinase Chk1)

CHK1, also known as CHEK1, it is a kinase that phosphorylates cdc25, an important phosphatase in cell cycle control, particularly for entry into mitosis. Cdc25, when phosphorylated on serine 216 by chk1 becomes bound by an adaptor protein in the cytoplasm. Therefore it is inhibited from removing the inhibiting phosphate from MPF (mitotic/maturation promoting factor) added by Wee1. Consequently, a cell is prevented from entering mitosis. Chk1 can also phosphorylate p53 at Ser20 in vitro.\n\nApplications:\nWestern Bloting: 1:500-1:2000.\nELISA: Suggested dilution 1:10000.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

030095

Size

100ul

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum, Mouse

Form

Purified antibody in PBS, 0.03% sodium azide.

P Type

Mab

Purity

Purified

Isotype

IgG1

References

1. EMBO J. 1998. 16: 545-554. \n2. Science 1998. 282: 1893-1897.

Additional Info

Human, Mouse

Alternative Names

CHEK1

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