Chk 1, phosphorylated (Ser280) (CHEK1, Cell Cycle Checkpoint Kinase, Serine/threonine Protein Kinase Chk1)

Chk1 is an evolutionarily conserved protein kinase that has been implicated in cell cycle checkpoint control in lower eukaryotes.Chk1 plays an essential role in the mammalian DNA damage checkpoint, embryonic development, and tumor suppression (1).It is activated in \nresponse to replication blocks and genotoxic stress, via phosphorylation of Serine 317 and Serine 345 (2). Activated Chk1 can bind to, phosphorylate (at Serine 216) and inactivate cdc25c (3, 4).Chk1 is phosphorylated by ATR in response to ultraviolet irradiation and inhibition of DNA replication, and by ATM in response to ionizing irradiation, resulting in enhanced kinase activity.Chk1 is phosphorylated at Serine 280 by AKT1/PKB, which is thought to promote mono and/or diubiquitination (5). \n \nApplications:\nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:2500-1:5000\nImmunoprecipitation: 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

C4200-15V

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 50mM Tris-Glycine (pH 7.4), 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide and 0.05% BSA.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Liu Q., et al. Genes Dev. 14:1448-1459, 2000 2. Zhao, H. et al. Mol. Cell. Biol. 21: 4129-4139, 2001 \n3. Zeng, Y., et al. Nature 395: 507-510, 1998 4. Sanchez, Y., et al. Science 277: 1497-501, 1997 \n5. The UniProt Consortium, The Universal Protein Resource (UniProt) Nucleic Acids Res. 36:D190-D195, 2008 \n

Additional Info

Recognizes human Chk1 when phosphorylated at Ser 280.

Alternative Names

CHEK1, Cell Cycle Checkpoint Kinase, Serine/threonine Protein Kinase Chk1