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Chk 1, phosphorylated (Ser280) (CHEK1, Cell Cycle Checkpoint Kinase, Serine/threonine Protein Kinase Chk1)

Chk 1, phosphorylated (Ser280) (CHEK1, Cell Cycle Checkpoint Kinase, Serine/threonine Protein Kinase Chk1)

Cat no: C4200-15V

Supplier: United States Biological
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Chk1 is an evolutionarily conserved protein kinase that has been implicated in cell cycle checkpoint control in lower eukaryotes.Chk1 plays an essential role in the mammalian DNA damage checkpoint, embryonic development, and tumor suppression (1).It is activated in response to replication blocks and genotoxic stress, via phosphorylation of Serine 317 and Serine 345 (2). Activated Chk1 can bind to, phosphorylate (at Serine 216) and inactivate cdc25c (3, 4).Chk1 is phosphorylated by ATR in response to ultraviolet irradiation and inhibition of DNA replication, and by ATM in response to ionizing irradiation, resulting in enhanced kinase activity.Chk1 is phosphorylated at Serine 280 by AKT1/PKB, which is thought to promote mono and/or diubiquitination (5). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:2500-1:5000 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Catalogue number: C4200-15V
Reactivities: Human
Hosts: Rabbit
Applications: Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as a liquid in 50mM Tris-Glycine (pH 7.4), 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide and 0.05% BSA.
P type: Mab
Isotype: IgG
Purity: Supernatant
Alternative names: CHEK1, Cell Cycle Checkpoint Kinase, Serine/threonine Protein Kinase Chk1
References: 1. Liu Q., et al. Genes Dev. 14:1448-1459, 2000 2. Zhao, H. et al. Mol. Cell. Biol. 21: 4129-4139, 2001 3. Zeng, Y., et al. Nature 395: 507-510, 1998 4. Sanchez, Y., et al. Science 277: 1497-501, 1997 5. The UniProt Consortium, The Universal Protein Resource (UniProt) Nucleic Acids Res. 36:D190-D195, 2008
Additional info: Recognizes human Chk1 when phosphorylated at Ser 280.

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