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Chk 1, phosphorylated (Ser296) (CHEK1, Cell Cycle Checkpoint Kinase, Serine/threonine Protein Kinase Chk1)

Cat no: C4200-15U

Chk 1, phosphorylated (Ser296) (CHEK1, Cell Cycle Checkpoint Kinase, Serine/threonine Protein Kinase Chk1)

Chk1 is a serine/threonine-protein kinase involved in cell cycle arrest when DNA damage has occurred, and it plays an important role in DNA damage checkpoint, embryonic development and tumor suppression. Chk1 is activated in response to replication blocks and genotoxic \nstress, via phosphorylation of serines 317 and 345 (2).Activated Chk1 can inactivate cdc25c through phosphorylation at Serine 216. Phosphorylation of cdc25c prevents activation of the CDC2-cyclin B complex and prevents entry into M-phase. \n \nApplications:\nSuitable for use in Western Blot and Immunocytochemistry/Immunofluorescence. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:1000-1:2000\nImmunocytochemistry/Immunofluorescence: 1:100-1:250\nOptimal dilutions to be determined by the researcher.\n\nPositive Controls:\n283T cell lysate treated with UV and AP; HeLa cells\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C4200-15U

Size

100ul

Applications

ICC, IF, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.05% BSA, 0.01% sodium azide, 50% glycerol

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Martinho, R.G., et al. EMBO J. 17: 7239-7249, 1998. 2. Zhao, H. and H. Piwnica-Worms. Mol. Cell. Biol. 21: 4129-4139, 2001. 3. Zeng, Y., et al. Nature 395: 507-510, 1998. 4. Sanchez, Y., et al. Science 277: 1497-501, 1997.

Additional Info

Recognizes human Chk1 when phosphorylated at Ser296 at ~54kD. Does not react with mouse or rat

Alternative Names

CHEK1, Cell Cycle Checkpoint Kinase, Serine/threonine Protein Kinase Chk1

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