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Chk2 (Checkpoint Kinase 2, Chek 2, Chek2,  Chk 2, CDS 1, CDS1, HuCds 1, HuCds1, LFS2, PP1425, RAD 53, RAD53, Serine/threonine Protein Kinase Chk2)

Cat no: C4200-90M

Chk2 (Checkpoint Kinase 2, Chek 2, Chek2, Chk 2, CDS 1, CDS1, HuCds 1, HuCds1, LFS2, PP1425, RAD 53, RAD53, Serine/threonine Protein Kinase Chk2)

Checkpoint homolog CHK2 is an important cell cycle checkpoint regulator. The protein is rapidly activated by phosphorylation in response to DNA damage and to replication block. When activated, CHK2 is known to prevent entry into mitosis and has been shown to regulate the tumor suppressor protein p53, leading to cell cycle arrest in G1. At least 12 isoforms are produced by alternative splicing.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested. \n\nRecommended Dilution:\nWestern Blot: 0.75ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C4200-90M

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Rat

Form

Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% Thimerosal, 0.05% sodium azide. Reconstitute with 100ul of sterile dH2O.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes human Checkpoint homolog CHK2 at 61kD. Species Crossreactivity: rat. Sequence Homology: mouse

Alternative Names

Checkpoint-like protein CHK2, CHK2 Checkpoint Homolog (S. pombe), EC=2.7.11.1, RP11-436C9.1

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