Chk2 (Checkpoint Kinase 2, Rad 53)

CHK2 controls cell cycle checkpoint and may participate in transduction of the DNA damage and replicational stress signals. CHK2 belongs to the Ser/Thr protein kinase family and its kinase actibity is up-regulated by autophosphorylation, It is phosphorylated rapidly in response to DNA damage and to replication block. \n\n\nCellular Localization: Nuclear\n\nPositive Control: HeLa, HepG2, MCF-7, or LN-Cap total cell extract.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C4200-90H

Size

100ug

Applications

WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2. No preservative added.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

Ahn J et al. Questioning the role of checkpoint kinase 2 in the p53 DNA damage response. J Biol Chem 278:20480-9 (2003). <PUBMED:12654916>Jallepalli PV et al. The Chk2 tumor suppressor is not required for p53 responses in human cancer cells. J Biol Chem 278:20475-9 (2003). <PUBMED:12654917>Stevens C et al. Chk2 activates E2F-1 in response to DNA damage. Nat Cell Biol 5:401-9 (2003). <PUBMED:12717439>Wu X & Chen J Autophosphorylation of checkpoint kinase 2 at serine 516 is required for radiation-induced apoptosis. J Biol Chem 278:36163-8 (2003). <PUBMED:12855706>Tan S et al. Zinc-finger protein-targeted gene regulation: genomewide single-gene specificity. Proc Natl Acad Sci U S A 100:11997-2002 (2003). <PUBMED:14514889>Lipton L et al. Contribution of the CHEK2 1100delC variant to risk of multiple colorectal adenoma and carcinoma. Cancer Lett 200:149-52 (2003). <PUBMED:14568168>Brody LC CHEKs and balances: accounting for breast cancer. Nat Genet 31:3-4 (2002). <PUBMED:11984555>

Additional Info

This product is specific for human Chk2 protein. Species Crossreactivity: Crossreacts with Human.

Alternative Names

CDS 1, CDS1, CHK2 checkpoint homolog, Checkpoint kinase 2, Chek 2, Chek2, Chk 2, HuCds 1, HuCds1, LFS2, PP1425, RAD 53, RAD53, Serine/threonine protein kinase Chk2, bA444G7