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Cholecystokinin 12 (CCK12) ELISA Kit

Cat no: ABIN1873490


Supplier: Antibodies-online
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Cholecystokinin 12 (CCK12) ELISA Kit: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to CCK12 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled CCK12 and unlabeled CCK12 (Standards or samples) with the pre-coated antibody specific to CCK12. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of CCK12 in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of CCK12 in the sample.
Catalogue number: ABIN1873490
Size: 96 tests
Antigen: Cholecystokinin 12 (CCK12)
Sensitivity: The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
Assay range: 12.35-1000 pg/mL
Sample type: Serum,Plasma,Biological Fluids

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