Cholesterol, Cholesteryl Ester, BioAssay(TM) Quantitation Kit II

Cholesterol is an essential molecule in all animal life. It has been involved in both normal\ndevelopment and diseases. The Cholesterol/Cholesteryl Ester Quantitation Kit provides a simple\nmethod for sensitive quantification of free cholesterol, cholesteryl esters, or both using a\ncolorimetric method. Most of the cholesterol in blood is in the form of cholesteryl ester. These\nesters can be hydrolyzed to free cholesterol under the appropriate conditions. In the assay, free\ncholesterol is oxidized by cholesterol dehydrogenase to generate NADH which reacts with a sensitive probe resulting in strong absorbance at 450nm. The assay can detect free or total cholesterol depending upon whether esterase is utilized to hydrolyze cholesterol esters present. Cholesteryl ester can be determined by subtracting the value of free cholesterol from the total cholesterol (cholesterol plus cholesteryl esters). The probe in this kit is more stable, sensitive and specific. The assay can tolerate interferences from various samples significantly.\n\nKit Components:\nCholesterol Assay Buffer 1x25ml\nSubstrate Mix (lyophilized) 1x1vial\nEnzyme Mix (lyophilized) 1x1vial\nEsterase (lyophilized) 1x1vial\nCholesterol Standard (2ug/ul) 1x100ul.\n\nStorage and Stability:\nStore at -20 degrees C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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