Choline Assay Kit, BioAssay(TM)

Choline and its metabolites play important roles in membrane structure integrity, cellular signaling and cholinergic neurotransmission. Aberrant regulation in choline metabolism has been associated with mental illness such as anxiety. This method provides a simple, direct and high-throughput assay for measuring choline in biological samples. In this assay, free choline is oxidized by choline oxidase to betaine and H2O2 which reacts with a specific dye to form a pink colored product. The color intensity at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the choline concentration in the sample.\n\nKey Features:\nUse 20ul samples. Linear detection range: Colorimetric Assay: 1 to 100uM, fluorimetric assay 0.2 to 10uM choline.\n\nApplications:\nAssays: choline in biological samples such as serum, plasma, urine, saliva, milk, tissue, and cell culture.\nDrug Discovery/Pharmacology: effects of drugs on choline metabolism.\n\nKit Contents:\nAssay Buffer: 10ml Enzyme Mix: 120ul\nDye Reagent: 120ul Standard: 400ul 2mM Choline\nStorage conditions. The kit is shipped on ice. Store all components at -20 degrees C. Shelf life of three months after receipt.\nPrecautions: reagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents. Please refer to Material Safety Data Sheet for detailed information.\n\nColorimetric Assay:\nSample treatment: liquid samples such as serum and plasma can be assayed directly. Tissue and cell lysates can be prepared by homogenization in cold 1 x PBS and centrifugation (5 min at 14,000rpm). Use clear supernatants for assay. Milk samples should be cleared by mixing 600ul milk with 100ul 6 N HCl. Centrifuge 5 min at 14,000 rpm. Transfer 300ul supernatant into a clean tube and neutralize with 50ul 6 N NaOH. The neutralized supernatant is ready for assay (dilution factor n=1.36).\nNote: (1). SH-containing reagents (e.g. b

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SPECIFICATIONS

Catalog Number

C5057-40

Size

1Kit

References

1. Lartillot, S. (1987). A simplified method of production of choline oxidase suitable for choline assay. Prep Biochem. 17:283-295.\n2. Gilberstadt, M.L. and Russell, J.A. (1984). Determination of picomole quantities of acetylcholine and choline in physiologic salt solutions. Anal Biochem. 138:78-85.\n3. Zeisel, S.H. and Millington, W.R. (1978). Free and choline assay. Am J Clin Nutr. 31:1978-1981.